Sex determination by amplification of amelogenin gene from dental pulp tissue by polymerase Chain Reaction

Sex determination of cattle meat by polymerase chain reaction amplification of the amelogenin gene

Aim: The objective of this study was to develop a simplified, efficient, and accurate protocol for sexing of cattle meat based on the amelogenin (AMELX/AMELY) gene using PCR technique which is superior to earlier work in terms of band patterns. Materials and Methods: Based on the amelogenin gene located on the conservation region of X and Y chromosomes, a pair of primers was designed and the sy...

SPECIFIC AMPLIFICATION OF ASPERGILLUS FUMlGATUS DNA BY POLYMERASE CHAIN REACTION

Invasive aspergillosis (1 is a life-threatening condition in immunocompromised patients. An early diagnosis is of great importance because early treatment may resolve this potentially fatal infection. Recently, the polymerase chain reaction (PCR) has been used successfully in detecting specific DNA of several pathogen. In this study, nested PCR was used to detect DNA specific for A!.pergiflus s...

Detection of the eaeA Gene in Escherichia coli Isolated from Broiler Chickens by Polymerase Chain Reaction

The aim of this study was to isolate Escherichia coli from chickens and to determine the presence of the eaeA gene, a virulence factor detected in Escherichia coli, in the isolates by polymerase chain reaction (PCR). Different chicken organs (lung, liver and spleen) were inoculated onto blood agar and biochemical tests were performed on the suspicious isolates. Escherichiacoliwas isolated from ...

specific amplification of aspergillus fumlgatus dna by polymerase chain reaction

invasive aspergillosis (1 is a life-threatening condition in immunocompromised patients. an early diagnosis is of great importance because early treatment may resolve this potentially fatal infection. recently, the polymerase chain reaction (pcr) has been used successfully in detecting specific dna of several pathogen. in this study, nested pcr was used to detect dna specific for a!.pergiflus s...

Detection of Amelogenin Region from Dental pulp by Loop-mediated Isothermal Amplification (LAMP)